歐柏榮 教

研究方向與計畫

一、骨骼肌肉的生長與發育:

(1)依鈣蛋白質分解酵素 (calpain) 在骨骼肌生長與分化所扮演的角色

(2)myostatin (growt/differentiation-8)對骨骼肌發育的影響

(3)鹿茸對動物骨骼肌肉生長與分化的影響

二、蛋殼形成相關基因之選殖與其調節:calbindin 及lysyl oxidase對蛋殼形成的影響。

三、土雞繁殖力功能性基因體研究。

四、蝦病毒檢驗試劑的研發。

近五年穫得之研究計畫:

九十二年度

(一) 提昇私立大學校院研發能量專案計畫:子計畫:細胞分化的分子機制 (III)(國科會)

(二) 豬與土機繁殖力表現體及cDNA晶片研發計畫子計畫:高繁殖力土雞表現序列株之建立(II)
(國科會)

(三)不同區域草蝦桿狀病毒去氧核甘酸序列斷定與分析(國科會)

九十一年度

(一) 提昇私立大學校院研發能量專案計畫:子計畫:細胞分化的分子機制 (II)(國科會)

(二) 禽纇殼腺黏膜細胞中與蛋殼品質相關cDNA基因庫之建立、基因篩選與利用(III) (農委會)

(三) PCR病毒檢測試劑冷凍乾燥以利運送及保存

(四) 豬與土機繁殖力表現體及cDNA晶片研發計畫子計畫:高繁殖力土雞表現序列株之建立(I)
(國科會)

九十年度

(一) 提昇私立大學校院研發能量專案計畫:

子計畫:細胞分化的分子機制(I) (國科會)

(二) 禽纇殼腺黏膜細胞中與蛋殼品質相關cDNA基因庫之建立、基因篩選與利用(II) (農委會)

(三) 第八生長分化因子基因分離定序與其生理功能的探討 (III) (國科會)

(四) 利用RT-PCR方法檢驗太平洋白蝦Taura syndrome (銳基國際股份有限公司)

八十九年度

(一) 第八生長分化因子基因分離定序與其生理功能的探討 (II) (國科會)

(二) 禽纇殼腺黏膜細胞中與蛋殼品質相關cDNA基因庫之建立、基因篩選與利用(I) (農委會)

八十八年度

(一) 第八生長分化因子基因分離定序與其生理功能的探討 (I) (國科會)

(二) 豬依鈣蛋白質分解酵素基因的分離與定序與其抗轉錄核醣核酸的表現 (農委會)

八十七年度

(一) Calpain 及protein kinase C 在肌肉細胞分化所扮演角色的探討 (國科會)

(二) 不同片段之依鈣蛋白質分解酵素抗轉錄核醣核酸對其轉殖動物生長的影響 (農委會)

八十六年度

(一) 大量表現活化u-calpain對肌肉細胞蛋白質分解的影響 (國科會)

(二) 蛋白質分解酵素抗轉錄核醣核酸在轉殖動物上之應用 (農委會)

 

論著發表

A. 期刊論文

1. Orwig, K., Bertrand, J. Ou, B.R., Forsberg, N. and Stromshak, F. 1994.
Involvement of protein kinase C, calpain and calpastatin in PGE2-alpha-induced
oxytocin secretion from the bovine corpus luteum. Endocrinology 134:78-83.

2. Liu, C. C., Yeh, J. Y., Ou, B. R. and Forsberg, N. E. 1994. Actions of
beta-adrenergic agonist on muscle protein metabolism in intack,
adrenalectomized and dexamethasone-supplemented adrenalectomized rats.
J. Nutri. Biochem. 5:43-49.

3. Yeh, J. Y., Ou, B. R. and Forsberg, N. E. 1994. Effects of dexamethasone on
muscle protein degradation and calpain and calpastatin activities and gene
expression in rabbits. J Endocrinology. 141:209-217.

4. Orwig, K., Bertrand, J. E., Ou, B. R., Forsberg, N. E. and Stromshak, F.
1994. Immunochemical characterization and cellular distribution of protein
kinase C isozyme in bovine corpus luteum. Comp. Biochem. Biophy. 108B:53-57.

5. Hong, D.H., Huan, J., Ou, B.R., Yeh, J.Y., Saido, T.C., Cheeke, P.R., Forsberg,
N.E., 1995, Protein kinase C isoforms in muscle cells and their regulation by
phorbol ester and calpain. Biochim. Biophys. Acta. 1267:45-54

6. Yeh, J. Y., Ou, B. R., Forsberg, N. E., Whanger, P. D. 1997. Effects of
selenium and serum on selenoprotein W in cultured L8 muscle cells. BioMetals 10:11-22.

7. Yeh, J. Y., Vendeland, S. C., Gu, Q. P., Butler, J. A., Ou, B. R., Whanger,
P. D. 1997. Dietary selenium increases selenoprotein W levels in rat tissues.
J. Nutrition. 127:2166-2172.

8. Yeh, J. Y., Ou, B. R., Gu, Q. P. and Whanger, P. D. 1998. Influence of
gender on selenoprotein W, glutathione peroxidase and selenium in tissue of
rats. Comparative Biochemistry and Physiology. 119B:151-155.

9. Sun, Y., P-C. Ha, J. A. Butler, B. R. Ou, J. Y. Yeh, and P. Whanger. 1998.
Effect of dietary selenium on selenoprotein W and glutathione peroxidase in 28
tissues of the rat. J. Nutr. Biochem. 9:23-27.

10. Ueda, Y., M. C. Wang, B. R. Ou, J. Huang, J. Elce, K. Tanaka, A. Ichihara,
N. E. Forsberg. 1998. Evidence of participation of the proteasome and
calpain in early phases of muscle cell differentiation. The international
Journal of Biochemistry and cell Biology. 30:679-694.

11. Ou, B. R., J. Huang, M. Ilian, D. H. Hong and N. E. Forsberg. 1999.
Genetic analysis of calpain isoformfunction in skeletal muscle: new
strategies to study protease function. In Calpain: Pharmacology and Toxicology of
calcium-dependent protease. Pp391-408. Taylor & Francis Co. PA. USA.

12. Horng, D. C., S. Y. Lai, H. W. Huang, Y. C. Liang and B. R. Ou. 2001.
Sequences of the 12S rRNA and the cytochrome b gene of the Formosan Serow
Naemorhedus swinhoei. Endemic Species Research 3:37-48.

13. Yeh, J. Y., L. C. Cheng, B. R. Ou, P. D. Whanger and L. W. Chang. 2002.
Differential influences of various arsenic compounds on glutathione redox
status and antioxidative enzymes in porcine endothelial cells. Cell. Mol.
Life Sci. 59: 1972-1982.
14. Horng, D. C., H. W. Huang, Y. C. Liang and B. R. Ou. 2003. Two distinct
phylogenetic groups of Formosan Serow (Naemorhedus swinhoei Gray)
population in Taiwan: base on mitochondrial D-loop region sequences Endemic
Species Resaerch. 5:15-25.

15. Liang, Y. C., J. Y. Yeh and B. R. Ou. 2003. Over-expression of porcine
growth/differentiation factor 8 recombinant protein in baculovirus expression system.
J. of Chinese Society of Animal Science 32: 23-32.

16. yeh, J. Y., L. C. Cheng, Y. C. Liang and B. R. Ou. 2003. Modulation of
the arsenic effects on cytotoxicity, cell viability and cell cycle in porcine
endothelial cells by selenium. Endothelial 10:127-139.

17. Han, G. H., B. R. Ou and S. K. Yang. 2003. Use of polymerase chain
reaction for sexing Formosa sika deer. J. of Chinese Society of Animal
Science 32:215-227.

18. Yeh, J. Y., L. C. Cheng, Y. C. Liang, B. R. Ou. 2003. Modulation of the
arsenic effects on cytotoxicity, viability, and cell cycle in porcine
endothelial cells by selenium. Endothelium 10:127-139.(按此下載檔案)

19. Lin, Y. J., B. R. Ou, C. C. Kuo. 2003. Quality characteristics of
duck breast muscle as affected by calcium chloride and post-mortem storage.
Taiwanese Journal of Agricultural Chemistry and Food Science 41:387-397.

20. Chung, T. C., Y. C. Liang, J. Y. Yeh, B. R. Ou. 2004. Stabilization of
calpain large subunits by overexpression of truncated calpain small s
ubunit in L8 myoblasts. Tissue & cell。Churchill Livingstone, Scotland。36:181-187。(按此下在檔案).

B.研討會論文

1. Ou, B. R., Hong, D. H. and Forsberg, N. E. 1994. Effects of calpain inhibitor,
chloroquine and IGF-1 on myofibrillar and non-myofibrillar protein
degradation and accretion in L8 myotubes. FASEB J. 8:A178.

2. Huan, J., Ou, B. R., Hong, D. H., Cheeke, P. R. and Forsberg, N. E. 1994.
Participation of calpains in phorbol ester-dependent down regulation of protein
kinase C isozymes in L8 myotubes. FASEB J. 8:A397.

3. 歐柏榮 1994. Effect of calpain inhibitor II, chloroquine and IGF-1 on muscle
protein degradation. 中畜會誌 (增刊) 23:61。

4. 劉建甫、許恭豪、歐柏榮、楊錫坤。1995。 梅花鹿泌乳素之cDNA選殖,
定序與基因之組織特異性。中畜會誌 (增刊) 24:140。

5. 葉貞吟、Whanger, P.、歐柏榮。1995。Effects of selenium and serum on
selenoprotein W and glutathione peroxidase in cultured L8 muscle cells.
中畜會誌(增刊) 24:141。

6. 葉貞吟、Whanger, P.、 歐柏榮。1996。 Differential expression of
selenoprotein W in male and female rats. 中畜會誌 (增刊) 25:134。

7. 鍾尊智、 歐柏榮。 1997。大量表現活化依鈣蛋白質分解酵素(calpain)
次單位對肌肉細胞蛋白質分解的影響。 中畜會誌 (增刊) 。26:77。

8. 高春成、歐柏榮、楊錫坤。1998。梅花鹿泌乳素基因之選殖。中畜會誌 (增刊) 27:62。

9. 梁佑全、歐柏榮。1998。利用依鈣蛋白質分解酵素之單股抗去氧核醣核酸對
肌肉細胞分化的影響。中畜會誌 (增刊) 27:120。

10. 柳源德、歐柏榮。1998。腫瘤殺死因子白介素-1及前列腺素E2對於肌肉蛋白質
分解之影響。中畜會誌( 增刊) 27:121。

11. 劉重威、柳源德、鍾尊智、陳思樺、梁佑全、歐柏榮。1998 。長期飢餓中大鼠
血清因子對肌肉蛋白質分解的影響。中畜會誌 (增刊) 27:122。

12. 韓嘉虹、歐柏榮、楊錫坤。1998。利用聚合脢連索反應判定梅花鹿之性別。
中畜會誌 (增刊) 27:123。
13. 陳瑞南、歐柏榮、楊錫坤。1999。鹿茸萃取液對梅花鹿初級培養鹿茸
細胞增殖之影響。中畜會誌 (增刊) 28:152。

14. 陳思樺、許恭豪、洪榮偉、梁佑全、歐柏榮。1999。表現依鈣蛋白質分解
酵素抗轉錄核醣核酸對其轉殖小鼠生長及體組成的影響。中畜會誌 (增刊)28:157。

15. 梁佑全、鄭敬薰、歐柏榮。2000。第八生長分化因子在大鼠骨骼肌原母細胞株
分化過程的表現。中畜會誌 (增刊)29:160。

16. 呂建和、陳怡君、歐柏榮。2000。菜鴨於產蛋期間calbindin基因於殼腺的表現。
中畜會誌 (增刊)29:161。
17. 陳思樺、洪榮偉、葉貞吟、歐柏榮。2000。Selenoprotein W之組織分佈與其於
不同年齡小鼠的變化。中畜會誌 (增刊)28:157。

18. Yeh, J. Y., L. C. Cheng, B. R. Ou and L. W. Chang. 2001. Effects of various
arsenic compounds on the proliferation of MDCK, C6 and L8 cell lines.
The international Symposium of Cellular Biology in Asia, Taipei, Tiwan. R. O. C.

19. Yeh, J. Y., L. C. Cheng, Y. H. Chang, L. W. Chang and B. R. Ou. 2002.
Differential influences of various arsenic compounds on glutathione redox
status and antioxidative enzymes. The 4th Asian-Pacific Organization for
Cell Biology Congress, Taipei, Taiwan, R. O. C.

20. Liang, Y. C., J. Y. Yeh and B. R. Ou. 2002. The role of porcccine
growth/differentiation factor 8 recombinant protein in muscle development.
J. Chin. Soc. Amin. Sci. 31:174.

21. Cheng, L. C., S. C. Yeh, B. R. Ou, Y. H. Cheng, L. W. Chang and
J. Y. Yeh. 2003. Arsenic extretionin porcine aortic endothelial cells.
The 18th Join Annual Conference of Biomedical Science, Taipei, Taiwan, R. O. C.

22.黃建德、鄧秉華、劉琳琳、葉貞吟、歐柏榮。2005。不同品系豬種之第八生長分化因子基因啟動子多樣性。中畜會誌(34):198。

23.陳明軒、陳思樺、許恭豪、鄧秉華、葉貞吟、歐柏榮。2005。利用精子載體法產製基因轉殖雞。中畜會誌(34):199。

24.曾柏澧、梁佑全、甯鈞慧、陳志峰、李淵百、葉貞吟、歐柏榮。2005。台灣土雞鐵蛋白質基因之選殖及其與產蛋性能之關係。中畜會誌(34):200。

25.江梅君、梁佑全、李孟修、歐柏榮、葉貞吟。2005。土雞含硒蛋白W的選殖與組織中的表現。中畜會誌(34):232。

26.Liang, Y.-C., J.-Y. Yeh, N.-E. Forsberg and B.-R. Ou. 2006. Involvement of μ- and m-calpains and protein kinase C isoforms in L8 myoblast differentiation. International Journal of Biochemistry and Cell Biology, 38: 662-670. (SCI)(按此下在檔案)

27.Yeh, J.-Y., B.-R. Ou, Y.-C. Liang, J. Burchfiel, J.-A. Butler, N.-E. Forsberg and P.-D. Whanger. 2006. Mechanism for proliferation inhibition by various selenium compounds and selenium-enriched broccoli extract in rat glial cells. Biometals, 19(6):611-621(SCI)(按此下載檔案).

28.Cheng, C.-H., B.-R. Ou, T.-F. SHen and S.-T. Ding. 2006. Effects of dietary Algal Docosahexaenoic acid oil supplement on fatty acid deposition and gene expression in laying Tsaiya ducks. Asian-Aust. J. Anim. Sci., 19(7): 1047-1053. (SCI)

29.Teng, P.-H., P.-Y. Lee, F.-C. Lee, H.-W. Chien, M.-S. Chen, P.-F. Sung, C. Su and B.-R. Ou. 2006. Detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Litopenaeus vannamei by ramification amplification assay. Disease of Aquatic Organisms, In press(SCI)

30.Huang, T.-Y., J.-Y. Yeh, B.-R. Ou and S.-K. Yang. 2006. The effect of antler extract on the antioxidative status in HepG2 cell line. The 21th Joint Annual Conference of Biomedical Science (生物醫學聯合學術年會)

31.Hsu, K. H., S.-H. Chen, J.-B. Wang, M.-S. Lee, J.-Y. Yeh, S.-D. Lee and B.-R Ou(歐柏榮).2006. Effect of exercise training on calpain systems in lean and obese Zucker rats. The 21th Joint Annual Conference of Biomedical Science (生物醫學聯合學術年會)

32.Yeh, J.-Y., J.-A. Butler, B.-R. Ou(歐柏榮), N.-E. Forsberg and P.-D Whanger. 2006. Mechanism for proliferation inhibition by various selenium compounds and selenium-enriched broccoli extract in rat embryonic fibroblasts. The 8th International Symposium on Selenium in Biology and Medicine, Wisconsin, USA.

33.Liang, Y. C., Yeh, J. Y. and Ou, B. R. 2007 Effect of maternal myostatin antibody on offspring
growth performance and body composition in mice. Journal of Experimental Biology. 210:477-483. (SCI)(按此下在檔案)

34.Tsai, S. H., Liu, Y. W., Tang W. C., Zhou, Z. W., Hwang, C. Y., Ou, B. R., Hu, C. P., Yang, V. C. And
Chen, J. K. 2007. Characterization of porcine arterial endothelial cells cultured on amniotic membrane, a potential matrix for vascular tissue engineering. Biochemical and Biophysical Research Communication. 15;357(4):984-90. (SCI)

35.Teng, P. H., Chen, C. L., Sung, P. F., Lee, F. C. Ou, B. R., and Lee, P. Y. 2007. Specific detection of reverse transcription-loop-mediated isothermal amplification amplicons for Taura syndrome virus by colorimetric dot-blot hybridization. Journal of Virological method 140:317-326. (SCI)

36.Ding, S. T., Ko, Y. H., Ou, B. R., Wang, P.H., Huang, M. C., Lee, Y. P., Lin, E.C., Chen, C. F., Lin, H.W. and Cheng W. T. K. 2008. The expression of genes related to egg production in the liver of Taiwanese country chickens. Asian-Aust. J. Anim. Sci. 21 (1) : 19 (SCI)

37.Yang, K. T., Lin, C. Y., Huang, H. L., Liou, J. S., Chien, C. Y., Wu, C. P., Huang, C. W., Ou, B. R., Chen, C. F., Lee, Y. P., Lin, Lin, E. C., Tang, P. C., Lee, W. C., Ding, S. T., Cheng, W. T. K., and Huang, M. C. 2008 Feb. Expressed transcripts associated with high rates of egg production in chicken ovarian follicles. Molecular cellular probes 22(1):47-54.

38.Cheng, Y. H., Ou, B. R., Cheng, L. C., Lu, J. H. and Yeh, J. Y.* (2008.12) Glutathione regulation in arsenic-induced porcine aortic endothelial cells. Toxicology in Vitro 22(8):1832-1839. (SCI)

 

 

 

 

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